Dig labelling kit manual

Digoxigenin Nick Translation Labeling Kit contains all reagents (except template and materials for purification of the probe) required for nick translation-based Digoxigenin labeling of DNA providing a highly efficient, easy-to-perform and rapid labeling technology. The labeling principle is similar to the DIG-Nick Translation Mix (Roche).  · Hi, I am having problems with the DIG labelling Kit. I am doing the protocol of T7 incubation for 2 hours ast 37°C, DNAse 15min at 37°C and precipitation with LiCl overnight at °C. PCR DIG Probe Synthesis Kit or PCR Labeling Mixes III. Nick-translation labeling of ds DNA with Nick Translation Mixes for in situ Probes 4 DIG Application Manual for In Situ Hybridization Table of Content Chapter 5 Procedures for In Situ Hybridization to Chromosomes.

DIG RNA Labeling Kit (SP6/T7) from Roche. Product Specs; Item DIG RNA Labeling Kit (SP6/T7) Company Roche; Catalog Number ; This product is no longer available on Biocompare. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Alexa Fluor® Protein Labeling Kit | 4 Calculate the degree of labeling: moles dye per mole protein = A × dilution factor where 71, cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® dye at nm. For IgGs, we find that labeling with moles of Alexa Fluor® dye per. The ANP NIDS® DIY Digoxigenin protein and antibody labeling Kits provide an easy way to label proteins and antibodies rapidly and efficiently. A simple, rapid quality control check is included in each kit to verify that the conjugation is successful. Compared to other methods, which often require at least µg of the conjugate, our QC.

Hi, I am having problems with the DIG labelling Kit. I am doing the protocol of T7 incubation for 2 hours ast 37°C, DNAse 15min at 37°C and precipitation with LiCl overnight at °C. Then I used DIG components for PCR reaction mix as recommended by Roche DIG manual including unlabelled DIG control and DIG label control provided with KIT. In addition to this I made separate. DIG RNA Labeling Kit (SP6/T7) From Roche Applied Science. During my work, I had to make RNA probes for in situ hybridization in Xenopus embryos and I used this kit. This kit allows the DNA template to be transcribed and cloned into the polylinker site of appropriate transcription vectors, which contain promoters for SP6 and/or T7 RNA polymerases.