Invitrogen gateway system manual

 · Here is an archived copy of the Invitrogen 3-insert multisite Gateway manual, version D, We originally had a live link to the Invitrogen site, but after this link broke it seemed easier to hard-archive a copy. Invitrogen Manual Version D(link updated August ) Note that Invitrogen seems to be continually tinkering with both att sequences and nomenclature, with no documentation . Life Technologies, a Division of Invitrogen Corporation www.doorway.ru Choosing Products to Build GATEWAY™ Expression Clones Step 1: Construct or Select an Entry Clone starting from: Design primers with attB sites Amplify PCR product Clone attB-PCR product into pDONR™ with BP CLONASE™ Enzyme Mix PCR Cloning System (BP CLONASE Mix included)File Size: KB. For more information on the Gateway® Technology, refer to the Gateway® Technology manual. This manual is available for downloading from our Web site (www.doorway.ru) or by contacting Technical Service (page 18). Experimental Outline To convert your vector to a destination vector, you will: 1. Choose the appropriate reading frame cassette to use.

Invitrogen™ Gateway™ Vector Conversion System with One Shot™. ccdB. Survival Cells. Designed to convert any vector into Gateway destination vector using restriction endonucleases and ligase. Manufacturer: Invitrogen™ Catalog No. $ / Each. Qty Check Availability. Here is an archived copy of the Invitrogen 3-insert multisite Gateway manual, version D, We originally had a live link to the Invitrogen site, but after this link broke it seemed easier to hard-archive a copy. Invitrogen Manual Version D (link updated August ). Invitrogen™ Gateway™ LR Clonase™ II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), the E. coli-encoded protein Integration Host Factor (IHF).

Gateway® 組換え反応 Gateway®テクノロジーは、λファージの組換えシステムを利用して、ベクタ ー間で、改変したatt 配列に挟まれたDNA 配列の交換反応を行う技術です （Hartley et al, ）。Gateway®テクノロジーは、以下に示す2 つの組換え反 応から構成されてい. Invitrogen Gateway recombination cloning uses a one hour reversible recombination reaction, without using restriction enzymes, ligase, subcloning steps, or screening of countless colonies, thereby saving you time, money, and effort. Benefits include: New advancements such as MultiSite Gateway Technology make Gateway cloning the ideal cloning method for protein expression and functional analysis. Life Technologies, a Division of Invitrogen Corporation www.doorway.ru Choosing Products to Build GATEWAY™ Expression Clones Step 1: Construct or Select an Entry Clone starting from: Design primers with attB sites Amplify PCR product Clone attB-PCR product into pDONR™ with BP CLONASE™ Enzyme Mix PCR Cloning System (BP CLONASE Mix included).